IgG1 kappa Isotype Control Fc fusion protein (FITC)

General information
 

Name:
IgG1 kappa Isotype Control Fc fusion protein (FITC)
Size:
100 ug
Catalog no:
35R-0010
Price:
340 EUR
 

Additional extra details
 

  • Research Area

    Immunology

    Product Subtype

    Isotype Controls

    Product Type

    Biological Reagents

  • Concentration

    500 ug/ml

    Form and Buffer

    aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.

    Storage

    Store at 2-8 deg C. Do not freeze. Protect from light.

  • Shipping Info

    Blue Ice

    Applications

    FC

    Tissue

    control

  • Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.

    Properties

    This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.

    Conjugation

    Anti-FITC Antibody

  • Group

    isotypes