PsbA, D1 positive control/quantitation standard

General information
 

Name:
PsbA, D1 positive control/quantitation standard
Size:
standard
Catalog no:
AS01 016S
Price:
295 EUR
 

Additional extra details
 

  • Available ordering format

    Inquire

    Immunogen

    to be determined

    Raised in

    N/A

  • Clonality

    N/A

    Clone

    N/A

    Purification

    Affinity purified

  • How to reconstitute

    For reconstitution add 85 µl of milliQ water.Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.

    Storage condition

    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    Verified applications

    western blot (WB)

  • Connected products

    collection of other protein standardsAS01 016 | anti-PsbA global hen antibodyAS05 084 | anti-PsbA global rabbit antibodyCollection of global antibodiesCollection of antibodies to photosynthetic proteins Plant protein extraction buffer

    Recommended dilutions for use

    Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a PsbA signal in this range.Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.Non-disulphie dependent dimers and complexes can be also detected using standard western blot methods with more sensitive detection reagents as ECL Advance or West Pico when loading per well more standard than recommended. They have not been included in the standard calibration.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

    Molecular weight (expected | аpparent)

    41.5 kDa (larger than native protein of 38 kDa due to the addition of His-tag). In most gel systems, PsbA migrates between 30 and 37 kDa

  • Verified reactivity

    to be determined

    Possible reactivity

    to be determined

    No reactivity

    to be determined

  • Supplementary information

    Concentration: after adding 85 µl of sterile milliQ water final concentration of the standard is 0.25 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

    References

    Vandenhecke et al. (2015). Changes in the Rubisco to photosystem ratio dominates photoacclimation across phytoplankton taxa. Photosynth Res. 2015 Apr 11. Wu et al. (2014). Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover rates. Front. Mar. Sci., 09 December 2014 | doi: 10.3389/fmars.2014.00068. Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.

    Scientific context

    The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.This is a recombinant protein standard, source: Synechocystis PCC 6803.

  • Notes

    The PsbA protein standard can be used in combination with global anti-PsbA antibodies to quantitate PsbA from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PsbA protein.Quantitative western blot: detailed method description, video tutorial

    Protein number

    Refer to NCBI

    TAIR number

    Refer to NCBI

  • Tissue

    control

    Description

    Isotype or positive controls by peptides, antibodies and deactivated samples.Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.

    Group

    positif