Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) (Western Blot Control)[Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)]

General information
 

Name:
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) (Western Blot Control)[Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)]
Size:
NA
Catalog no:
MBS343038
Price:
5 EUR
 

Additional extra details
 

  • Products_type

    Western Blot Control

    Products_gene_name

    [GM-CSF]

    Tissue

    control, granulocyte

  • Test

    Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.

    Description

    Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard.Isotype or positive controls by peptides, antibodies and deactivated samples.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

    Gene

    Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.